Anti-SYT2 antibody [EPR13204] (ab181123)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13204] to SYT2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-SYT2 antibody [EPR13204]
See all SYT2 primary antibodies -
Description
Rabbit monoclonal [EPR13204] to SYT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human cerebellum lysate, Rat and Mouse brain tissue. His-tagged mouse recombinant protein SYT2 (aa137-420).
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13204 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-SYT2 antibody [EPR13204] (ab181123) at 1/1000 dilution
Lane 1 : His-tagged mouse recombinant protein SYT1 (aa137-421)
Lane 2 : His-tagged mouse recombinant protein SYT2 (aa137-420)
Lane 3 : His-tagged mouse recombinant protein SYT3 (aa 76-587)
Lane 4 : His-tagged mouse recombinant protein SYT4 (aa 38-425)
Lane 5 : His-tagged mouse recombinant protein SYT5 (aa 46-386)
Lane 6 : His-tagged mouse recombinant protein SYT6 (aa 81-511)
Lane 7 : His-tagged mouse recombinant protein SYT7 (aa 38-403)
Lane 8 : His-tagged mouse recombinant protein SYT9 (aa 74-491)
Lysates/proteins at 0.03 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 47 kDaThis antibody has no cross-reacivity with mouse SYT1 , SYT3, SYT4, SYT5, SYT6, SYT7, and SYT9. These rec proteins were both made in house and extracted from E.coil expression systems.
-
All lanes : Anti-SYT2 antibody [EPR13204] (ab181123) at 0.628 µg/ml
Lane 1 : Mouse Brain Tissue
Lane 2 : Rat Brain Tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg
Predicted band size: 47 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST .
-
Anti-SYT2 antibody [EPR13204] (ab181123) at 1/5000 dilution + Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
-