Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16789] to RON - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RON antibody [EPR16789] - BSA and Azide free
    See all RON primary antibodies

  • Description

    Rabbit monoclonal [EPR16789] to RON - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251281 is the carrier-free version of ab199532. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251281 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
    Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
    All lanes : Anti-RON antibody [EPR16789] (ab199532) at 1/4000 dilution

    Lane 1 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 152 kDa
    Observed band size: 185 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab199532, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 17616662).

  • Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
    Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
    All lanes : Anti-RON antibody [EPR16789] (ab199532) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : MDA-MB-435S (Human ductal carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 152 kDa


    Exposure time: 3 minutes


    This data was developed using ab199532, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Both MCF7 and MDA MB 435S are RON negative cell lines.

  • Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
    Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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