Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [LpMab-7] to Podoplanin / gp36 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free
    See all Podoplanin / gp36 primary antibodies

  • Description

    Mouse monoclonal [LpMab-7] to Podoplanin / gp36 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: U-2 OS cell lysate. IHC-P: Human mesothelioma, Human placenta tissues.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256566 is the carrier-free version of ab256561. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Podoplanin / gp36 with ab256561 at 1/10000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP). Positive staining on the vessels in the human placenta. The section was incubated with ab256561 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256561).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)

    Immunohistochemical analysis of paraffin-embedded Human mesothelioma tissue labeling Podoplanin / gp36 with ab256561 at 1/10000 dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP). Membranous staining on human mesothelioma. The section was incubated with ab256561 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256561).

  • Western blot - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)
    Western blot - Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)
    All lanes : Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561) at 1/1000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 16 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST The molecular weight observed is consistent with what has been described in the literature (PMID: 25080943; PMID: 26090595; PMID: 26416352).

    Negative control: HUVEC (PMID: 21920451)

    Exposure time: 20 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256561).

  • Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)
    Anti-Podoplanin / gp36 antibody [LpMab-7] - BSA and Azide free (ab256566)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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