Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1466Y] to STK3/MST-2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free

  • Description

    Rabbit monoclonal [EP1466Y] to STK3/MST-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Wildtype HAP1 cells, NIH/3T3 (Mouse embryonic fibroblast) cells; IHC-P: Human lymphoma tissue; Flow Cyt: NIH/3T3 (Mouse embryonic fibroblast).

  • General notes

    ab232323 is the carrier-free version of ab52641. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232323 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1466Y

  • Isotype

    IgG

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Immunocytochemistry/ Immunofluorescence - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

    ab52641 staining STK3 in wild-type HAP1 cells (top panel) and STK3 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab52641 at 5μg/ml concentration and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52641).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab52641 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52641).

  • Flow Cytometry - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Flow Cytometry - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

    NIH/3T3 (Mouse embryonic fibroblast) cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol.  The primary antibody (ab52641) was used at a 1:70 dilution (1 μg) (red).  A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1:2000 dilution.  A Rabbit monoclonal IgG (ab172730) (black) was used as an isotype control. Cells without incubation with primary antibody and secondary antibody were used as an unlabelled control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52641).

  • Immunoprecipitation - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Immunoprecipitation - Anti-STK3 / MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

    ab52641 (purified) at 1/50 immunoprecipitating STK3 / MST-2 in 10 μg C6 cell lysate (Lanes 1 and 2, observed at 56 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52641).

  • Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Immunocytochemistry/ Immunofluorescence - Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

    Confocal image showing cytoplasmic staining of STK3/MST-2 in NIH/3T3 (mouse embryonic fibroblast) cells using ab52641 . The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. The cells were then incubated with ab52641 at 1/70 dilution and counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/100 dilution (red). Goat anti Rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52641).

  • Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)
    Anti-STK3/MST-2 antibody [EP1466Y] - BSA and Azide free (ab232323)

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