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Cancer Cell cycle Cell cycle inhibitors Ink4

Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)

Price and availability

526 012 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a - BSA and Azide free
  • Suitable for: IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free
    See all CDKN2A/p16INK4a primary antibodies
  • Description

    Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Mouse CDKN2A/p16INK4a. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q64364

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Wild type mouse fibrosarcoma. Mouse papilloma tissue.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252788 is the carrier-free version of ab241543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    PABLO33B
  • Isotype

    IgG2a
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf
    • Signal Transduction
    • Antibodies
    • cdkn2a/p16ink4a

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)

    Image A: Positive staining on the wild-type mouse fibrosarcoma.

    Image B: No staining on the p16 knock-out mouse fibrosarcoma.

    The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1:160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Paraffin-embedded sections. Counterstained with hematoxylin.

    The image was kindly provided by Spanish National Cancer Research Centre (CNIO).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)

    Positive staining on paraffin-embedded mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1:160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with hematoxylin.

    The image was kindly provided by Spanish National Cancer Research Centre (CNIO).

  • Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
    Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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