Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
![Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)](https://www.abcam.com/ps/products/252/ab252788/Images/ab252788-393162-anti-cdkn2a-p16ink4a-antibody-pablo33b-immunohistochemistry-fibrosarcoma-mouse.jpg "Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a - BSA and Azide free
- Suitable for: IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free
See all CDKN2A/p16INK4a primary antibodies -
Description
Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Mouse CDKN2A/p16INK4a. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q64364 -
Positive control
- IHC-P: Wild type mouse fibrosarcoma. Mouse papilloma tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab252788 is the carrier-free version of ab241543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
PABLO33B -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)
Image A: Positive staining on the wild-type mouse fibrosarcoma.
Image B: No staining on the p16 knock-out mouse fibrosarcoma.
The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1:160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Paraffin-embedded sections. Counterstained with hematoxylin.
The image was kindly provided by Spanish National Cancer Research Centre (CNIO).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)](https://www.abcam.com/ps/products/252/ab252788/Images/ab252788-390084-AntiCDKN2A-p16INK4a-PABLO33B-BSA-Azide-free-immunohistochemistry-papilloma-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)Positive staining on paraffin-embedded mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The dilution of the recombinant antibody was 1:160 and the secondary antibody was a Rabbit a-Rat Biotinylated followed by OmniMap anti rabbit. Counterstained with hematoxylin.
The image was kindly provided by Spanish National Cancer Research Centre (CNIO).
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![Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)](https://www.abcam.com/ps/products/252/ab252788/Images/ab252788-4-benefits-of-recombinant-antibodies.png)
Anti-CDKN2A/p16INK4a antibody [PABLO33B] - BSA and Azide free (ab252788)