Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP271] to CDKN2A/p14ARF - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free
    See all CDKN2A/p14ARF primary antibodies

  • Description

    Rabbit monoclonal [SP271] to CDKN2A/p14ARF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human cervical squamous cell carcinoma tissue. WB: HeLa cell lysate. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HEK293 and HeLa cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab271999 is the carrier-free version of ab216602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
    Flow Cytometry - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF with purified ab216602 at 1/420 dilution (1.02 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).
  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling CDKN2A/p14ARF  with purified ab216602 at 1/500 (0.86 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma labeling CDKN2A/p14ARF with ab216602 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).
  • Flow Cytometry - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
    Flow Cytometry - Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

    Flow cytometric analysis of HEK293 cells labeling CDKN2A/p14ARF with ab216602 at 1/400 dilution (green) compared to negative control rabbit IgG (blue) .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216602).
  • Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)
    Anti-CDKN2A/p14ARF antibody [SP271] - BSA and Azide free (ab271999)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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