Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8848] to BAF57/SMARCE1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, ChIP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free
    See all BAF57/SMARCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8848] to BAF57/SMARCE1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ChIPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and MCF7 cell lysates. ChIP: HeLa cells.

  • General notes

    Ab248408 is the carrier-free version of ab131328. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248408 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)
    Western blot - Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)
    All lanes : Anti-BAF57/SMARCE1 antibody [EPR8848] (ab131328) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SMARCE1 knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 47 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab131328).

    Lanes 1-3: Merged signal (red and green). Green - ab131328 observed at 51 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab131328 Anti-BAF57/SMARCE1 antibody [EPR8848] - ChIP Grade was shown to specifically react with BAF57/SMARCE1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265780 (knockout cell lysate ab258200) was used. Wild-type and BAF57/SMARCE1 knockout samples were subjected to SDS-PAGE. ab131328 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • ChIP - Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)
    ChIP - Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)

    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab131328 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131328).

  • Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)
    Anti-BAF57/SMARCE1 antibody [EPR8848] - BSA and Azide free (ab248408)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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