Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14335-78] to SOX9 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free
    See all SOX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR14335-78] to SOX9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SW480, PC3 cells and cell lysates; Human breast carcinoma tissue; Human, Mouse and Rat colon tissues, pig small intestine tissue. ICC/IF: F9 cells.

  • General notes

    Ab225541 is the carrier-free version of ab185966. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab225541 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    ab185966 staining SOX9 in developing eye of mouse tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/1000 in PBS) at 21°C for 4 hours. A Biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded pig small intestine sections labelling SOX9 with ab185966 at dilution of 1/2000. The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

    The image shows intense enterocyte/goblet cell nuclear positivity, confined to the crypts of Lieberkühn.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunofluorescence analysis of 4% paraformaldehyde fixed SW480 cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. Dapi staining shown in blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    Immunofluorescence analysis of 4% paraformaldehyde fixed PC3 cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. Dapi staining shown in blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185966).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

    This ICC data was generated using the same anti-SOX9 antibody clone [EPR14335-78] in a different buffer formulation (cat# ab185966).

    ab185966 staining Sox9 in F9 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5μg/ml concentration and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1μg/ml concentration, followed by a further incubation at room temperature for 1h with an anti-rabbit AlexaFluor® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
    Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)

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