Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SOX9 antibody [EPR12755] (ab182579)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12755] to SOX9
Suitable for: Flow Cyt, WB, ICC/IF, IP
Reacts with: Human

Product name

Anti-SOX9 antibody [EPR12755]
See all SOX9 primary antibodies
Description

Rabbit monoclonal [EPR12755] to SOX9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- SW480 and Caco 2 cell lysates; SW480 and Caco 2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12755
Isotype

IgG
Research areas

Western blot - Anti-SOX9 antibody [EPR12755] (ab182579)

All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/10000 dilution

Lane 1 : SW480 cell lysate
Lane 2 : Caco 2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunoprecipitation - Anti-SOX9 antibody [EPR12755] (ab182579)

All lanes : Anti-SOX9 antibody [EPR12755] (ab182579) at 1/80 dilution

Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate, 10ug
Lane 2 : SW480, 350ug, +, ab182579 2ug
Lane 3 : SW480 cell lysate, 350ug + rabbit IgG (ab172730) , 2ug

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody (HRP))

Observed band size: 75 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] (ab182579)

Immunofluorescent analysis of Caco 2 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Flow Cytometry - Anti-SOX9 antibody [EPR12755] (ab182579)

Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma ) cells labeling SOX9 with purified ab182579 at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] (ab182579)

Immunofluorescence analysis of SW480 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.
Immunoprecipitation - Anti-SOX9 antibody [EPR12755] (ab182579)

Detection of SOX9 by Western Blot of Immunprecipitate.
Caco 2 cell lysate immunoprecipitated using ab182579 at 1/50 dilution.
A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Anti-SOX9 antibody [EPR12755] (ab182579)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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