Anti-SOX9 (phospho S181) antibody (ab59252)
Key features and details
- Rabbit polyclonal to SOX9 (phospho S181)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOX9 (phospho S181) antibody
See all SOX9 primary antibodies -
Description
Rabbit polyclonal to SOX9 (phospho S181) -
Host species
Rabbit -
Specificity
Detects endogenous levels of SOX9 only when phosphorylated at serine 181. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SOX9. Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).
Database link: P48436 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with UV
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDaLoading control = GAPDH
10% SDS-PAGE
Primary incubation: 4°C overnight
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ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution
Lane 1 : 293 cell extracts treated
with PBS (60mins)
Lane 2 : 293 cell extracts treated
with PBS (60mins) with immunizing phospho-peptide
Predicted band size: 56 kDa
Observed band size: 56 kDa