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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box

Anti-SOX9 (phospho S181) antibody (ab59252)

Price and availability

308 236 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-SOX9 (phospho S181) antibody (ab59252)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SOX9 (phospho S181)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SOX9 (phospho S181) antibody
    See all SOX9 primary antibodies
  • Description

    Rabbit polyclonal to SOX9 (phospho S181)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of SOX9 only when phosphorylated at serine 181.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SOX9. Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).
    Database link: P48436

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Developmental Biology
    • Reproduction
    • Sex determination
    • Developmental Biology
    • Reproduction
    • Placental development
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone

Images

  • Western blot - Anti-SOX9 (phospho S181) antibody (ab59252)
    Western blot - Anti-SOX9 (phospho S181) antibody (ab59252)
    All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate treated with UV

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution

    Predicted band size: 56 kDa



    Loading control = GAPDH

    10% SDS-PAGE

    Primary incubation: 4°C overnight

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 (phospho S181) antibody (ab59252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 (phospho S181) antibody (ab59252)
    ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • Western blot - Anti-SOX9 (phospho S181) antibody (ab59252)
    Western blot - Anti-SOX9 (phospho S181) antibody (ab59252)
    All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution

    Lane 1 : 293 cell extracts treated
    with PBS (60mins)
    Lane 2 : 293 cell extracts treated
    with PBS (60mins) with immunizing phospho-peptide

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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