Anti-BBX antibody [2065C12a] (ab50824)
![Anti-BBX antibody [2065C12a] (ab50824)](https://www.abcam.com/ps/products/50/ab50824/Images/ab50824-62516-anti-bbx-antibody-2065c12a-western-blot.jpg "Anti-BBX antibody [2065C12a] (ab50824)")
Key features and details
- Mouse monoclonal [2065C12a] to BBX
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-BBX antibody [2065C12a]
See all BBX primary antibodies -
Description
Mouse monoclonal [2065C12a] to BBX -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- HeLa cells or 3T3 cells nuclear or whole celle lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50824 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter sterilized. -
Clonality
Monoclonal -
Clone number
2065C12a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-BBX antibody [2065C12a] (ab50824)Anti-BBX antibody [2065C12a] (ab50824) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-BBX antibody [2065C12a] (ab50824)](https://www.abcam.com/ps/products/50/ab50824/Images/ab50824-62515-anti-bbx-antibody-2065c12a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BBX antibody [2065C12a] (ab50824)Immunofluorescent staining of BBX expression HeLa cells using ab50824 at a 1/50 dilution and and AlexaFluor 488 conjugated goat anti-mouse secondary
