Anti-BAF57/SMARCE1 antibody (ab228750)
Key features and details
- Rabbit polyclonal to BAF57/SMARCE1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-BAF57/SMARCE1 antibody
See all BAF57/SMARCE1 primary antibodies -
Description
Rabbit polyclonal to BAF57/SMARCE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human BAF57/SMARCE1 (internal sequence). The exact sequence is proprietary.
Database link: Q969G3 -
Positive control
- WB: NIH/3T3, IMR32 and Jurkat whole cell lysates. IHC-P: NCI-H1299 xenograft tissue. ICC/IF: MCF7 and HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BAF57/SMARCE1 antibody (ab228750)Anti-BAF57/SMARCE1 antibody (ab228750) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 47 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-BAF57/SMARCE1 antibody (ab228750)All lanes : Anti-BAF57/SMARCE1 antibody (ab228750) at 1/1000 dilution
Lane 1 : IMR32 whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (ab228750)Paraffin-embedded NCI-H1299 (human) xenograft tissue stained for BAF57/SMARCE1 with ab228750 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody (ab228750)4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for BAF57/SMARCE1 (green) using ab228750 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody (ab228750)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BAF57/SMARCE1 (green) using ab228750 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). Alpha Tubulin, a cytoskeleton marker, is detected with an alpha Tubulin antibody at 1/500 dilution (red).
