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Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8849] to BAF57/SMARCE1 - ChIP Grade
  • Suitable for: ChIP, WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade
    See all BAF57/SMARCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8849] to BAF57/SMARCE1 - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BAF57/SMARCE1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • MCF7, HeLa, Jurkat and Raji cell lysates; Human brain tissue; MCF7 cells. IP: MCF7 cell lysate Flow Cyt: MCF7 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    All lanes : Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081) at 1/1000 dilution

    Lane 1 : MCF7 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : Jurkat cell lysates
    Lane 4 : Raji cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    ab137081 (purified) at 1/20 dilution immunoprecipitating BAF57/SMARCE1 in MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg.

    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab137081 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137081 in MCF7 whole cell lysate

    For western blotting, ab137081 at 1/500 dilution (0.02 µg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

  • Flow Cytometry - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Flow Cytometry - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BAF57/SMARCE1 with purified ab137081 at 1/100 dilution (10.38 µg/mL) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlabeled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab137081 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  • Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Immunofluorescent analysis of MCF7 cells labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.
  • Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)
    Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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