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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-NUP50 antibody (ab85915)

Anti-NUP50 antibody (ab85915)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NUP50
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NUP50 antibody
    See all NUP50 primary antibodies
  • Description

    Rabbit polyclonal to NUP50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 418 and 468 of Human NUP50 (NP_009103.2)

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab85915 was affinity purified using an epitope specific to NUP50 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Images

  • Western blot - Anti-NUP50 antibody (ab85915)
    Western blot - Anti-NUP50 antibody (ab85915)
    All lanes : Anti-NUP50 antibody (ab85915) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate. at 50 µg
    Lane 2 : HeLa whole cell lysate. at 15 µg
    Lane 3 : HeLa whole cell lysate. at 5 µg

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody (ab85915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody (ab85915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling NUP50 with ab85915 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-NUP50 antibody (ab85915)
    Immunoprecipitation - Anti-NUP50 antibody (ab85915)
    Detection of NUP50 by Western Blot of Immunprecipitate.
    ab85915 at 1µg/ml staining NUP50 in HeLa whole cell lysate immunoprecipitated using ab85915 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NUP50 antibody (ab85915)

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    Anti-NUP50 antibody [EPR9526] - BSA and Azide free (ab240104)

    Applications: ICC/IF, WB

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    Anti-NUP50 antibody [EPR9525] (ab151567)

    Applications: ICC/IF, IHC-P, WB

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    Anti-NUP50 antibody [EPR9525] - BSA and Azide free (ab240121)

    Applications: ICC/IF, IHC-P, WB

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    Anti-NUP50 antibody [EPR9526] (ab137092)

    Applications: ICC/IF, WB

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