Anti-NUP50 antibody [EPR9526] (ab137092)
![Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151676-anti-nup50-antibody-epr9526-western-blot.jpg "Anti-NUP50 antibody [EPR9526] (ab137092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9526] to NUP50
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-NUP50 antibody [EPR9526]
See all NUP50 primary antibodies -
Description
Rabbit monoclonal [EPR9526] to NUP50 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.
-
Positive control
- Jurkat, HeLa and 293T cell lysates; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9526 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
-
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151675-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151674-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092) This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview. -
![Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-4-benefits-of-recombinant-antibodies.png)
Anti-NUP50 antibody [EPR9526] (ab137092)
