Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NUP50 antibody [EPR9525] (ab151567)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9525] to NUP50
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-NUP50 antibody [EPR9525]
See all NUP50 primary antibodies
Description

Rabbit monoclonal [EPR9525] to NUP50
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human NUP50 aa 1-100. The exact sequence is proprietary.
Positive control
- Jurkat, HeLa, 293T and HepG2 cell lysates; Human colon tissue; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9525
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)

All lanes : Anti-NUP50 antibody [EPR9525] (ab151567) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUP50 with ab151567 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunofluorescent analysis of HeLa cells labeling NUP50 with ab151567 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-NUP50 antibody [EPR9525] (ab151567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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