Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614]
    See all Hemoglobin subunit beta/ba1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: TF-1 cell lysate; Human lung and fetal heart lysates; Rat heart lysate; Mouse heart and lung lysates. IP: TF-1 cell lysate.

  • General notes

     This product was previously labelled as Hemoglobin subunit beta

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20614

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : His-tagged human hemoglobin subunit beta/ba1 (aa1-147) recombinant protein
    Lane 2 : His-tagged human hemoglobin subunit delta (aa2-147) recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Positive Control: Anti-His-tag® antibody (below)

  • Immunoprecipitation - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Immunoprecipitation - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

    Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: TF-1 whole cell lysate 10 µg (Input). 

    Lane 2: ab214049 IP in TF-1 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

    Lane 1 : His-tagged human hemoglobin subunit epsilon (aa1-147) recombinant protein
    Lane 2 : His-tagged human hemoglobin subunit gamma-2 (aa1-147) recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Positive Control: Anti- 6X His-tag® antibody ab213204 (below)

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

    Lane 1 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Positive Control: Anti-GAPDH antibody ab181602 (below)

    It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate at 10 µg
    Lane 2 : Human lung lysate at 20 µg

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Lanes 1 & 3 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution
    Lane 2 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

    Lane 1 : Rat heart lysate at 20 µg
    Lane 2 : Mouse heart lysate at 20 µg
    Lane 3 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.

  • Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)
    Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

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