Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614]
See all Hemoglobin subunit beta/ba1 primary antibodies
Description

Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: TF-1 cell lysate; Human lung and fetal heart lysates; Rat heart lysate; Mouse heart and lung lysates. IP: TF-1 cell lysate.
General notes
This product was previously labelled as Hemoglobin subunit beta

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20614
Isotype

IgG
Research areas

Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

Lane 1 : His-tagged human hemoglobin subunit beta/ba1 (aa1-147) recombinant protein
Lane 2 : His-tagged human hemoglobin subunit delta (aa2-147) recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Positive Control: Anti-His-tag^®antibody (below)
Immunoprecipitation - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1: TF-1 whole cell lysate 10 µg (Input).

Lane 2: ab214049 IP in TF-1 whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

Lane 1 : His-tagged human hemoglobin subunit epsilon (aa1-147) recombinant protein
Lane 2 : His-tagged human hemoglobin subunit gamma-2 (aa1-147) recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Positive Control: Anti- 6X His-tag^®antibody ab213204 (below)
Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

Lane 1 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Positive Control: Anti-GAPDHantibody ab181602 (below)

It has been shown that hemoglobin subunit beta/ba1 is not expressed in HEK-293 cells.
Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

Lane 1 : Human fetal heart lysate at 10 µg
Lane 2 : Human lung lysate at 20 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

Lanes 1 & 3 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution
Lane 2 : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/10000 dilution

Lane 1 : Rat heart lysate at 20 µg
Lane 2 : Mouse heart lysate at 20 µg
Lane 3 : Mouse lung lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.
Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049)

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