Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
![Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-170583-ab170954WB1.jpg "Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11934(B)] to PNK/PNKP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-PNK/PNKP antibody [EPR11934(B)]
See all PNK/PNKP primary antibodies -
Description
Rabbit monoclonal [EPR11934(B)] to PNK/PNKP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PNK/PNKP aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96T60 -
Positive control
- HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.
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General notes
This product was previously labelled as PNK
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11934(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)All lanes : Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-221489-ab170954ihcp3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)ab170954 showing +ve staining in Human testis tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-221488-ab170954ihcp2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)ab170954 showing +ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-221487-ab170954ihcp1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-170586-ab170954IHC21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
The use of an HRP/AP polymerized secondary antibody is recommended.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-170585-ab170954IHC11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
The use of an HRP/AP polymerized secondary antibody is recommended.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-170584-ab170954IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.
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![Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)](https://www.abcam.com/ps/products/170/ab170954/Images/ab170954-11-benefits-of-recombinant-antibodies.png)
Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
