Recombinant human Peroxiredoxin 2/PRP protein (ab79947)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
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Product name
Recombinant human Peroxiredoxin 2/PRP protein
See all Peroxiredoxin 2/PRP proteins and peptides -
Biological activity
Specific Activity: 149 pmol/min/µg. 3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min. -
Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)
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SDS-PAGE - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)Lane 1: ab79947 on 14% SDS-PAGE, Coomassie staining, 10µg.
Lane 2: Protein marker. -
Western blot - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)
