Anti-eIF4A2 antibody (ab228693)
Key features and details
- Rabbit polyclonal to eIF4A2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4A2 antibody
See all eIF4A2 primary antibodies -
Description
Rabbit polyclonal to eIF4A2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human eIF4A2 (internal sequence). The exact sequence is proprietary.
Database link: Q14240 -
Positive control
- WB: HeLa and HepG2 whole cell extracts. IHC-P: Human ovarian carcinoma tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4A2 antibody (ab228693)All lanes : Anti-eIF4A2 antibody (ab228693) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-eIF4A2 antibody (ab228693)Methanol-fixed A431 (Human epidermoid carcinoma cell line) cells stained for eIF4A2 (green) using ab228693 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A2 antibody (ab228693)Paraffin-embedded human ovarian carcinoma tissue stained for eIF4A2 using ab228693 at 1/500 dilution in immunohistochemical analysis.
