Anti-eIF4A2 antibody (ab31218)
Key features and details
- Rabbit polyclonal to eIF4A2
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4A2 antibody
See all eIF4A2 primary antibodies -
Description
Rabbit polyclonal to eIF4A2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human eIF4A2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32473) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IP (1) Use at an assay dependent concentration.WB (4) Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Notes IP
Use at an assay dependent concentration.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Target
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Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. -
Sequence similarities
Belongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 1974 Human
- Entrez Gene: 13682 Mouse
- Entrez Gene: 303831 Rat
- Omim: 601102 Human
- SwissProt: Q14240 Human
- SwissProt: P10630 Mouse
- SwissProt: Q5RKI1 Rat
- Unigene: 518475 Human
see all -
Alternative names
- ATP dependent RNA helicase eIF4A 2 antibody
- ATP-dependent RNA helicase eIF4A-2 antibody
- BM-010 antibody
see all
Images
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Western blot - Anti-eIF4A2 antibody (ab31218)Anti-eIF4A2 antibody (ab31218) at 1 µg/ml +
Jurkat whole cell lysate (ab7899) at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-eIF4A2 antibody (ab31218)eIF4A2 was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to eIF4A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 47kDa: eIF4A2 . -
Western blot - Anti-eIF4A2 antibody (ab31218)All lanes : Anti-eIF4A2 antibody (ab31218) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Ovary (Mouse) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lane 8 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab31218 has been referenced in 23 publications.
- Wertz MH et al. Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity. Neuron 106:76-89.e8 (2020). PubMed: 32004439
- Lyu S et al. High expression of eIF4A2 is associated with a poor prognosis in esophageal squamous cell carcinoma. Oncol Lett 20:177 (2020). PubMed: 32934744
- Zhang J et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). PubMed: 31015415
- Iwasaki S et al. The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA. Mol Cell 73:738-748.e9 (2019). PubMed: 30595437
- Wilczynska A et al. eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR. Genome Biol 20:262 (2019). PubMed: 31791371
Images
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Western blot - Anti-eIF4A2 antibody (ab31218)Anti-eIF4A2 antibody (ab31218) at 1 µg/ml +
Jurkat whole cell lysate (ab7899) at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-eIF4A2 antibody (ab31218)
eIF4A2 was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to eIF4A2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 47kDa: eIF4A2 . -
Western blot - Anti-eIF4A2 antibody (ab31218)All lanes : Anti-eIF4A2 antibody (ab31218) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Ovary (Mouse) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Brain (Rat) Tissue Lysate
Lane 8 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
