Human eIF4E ELISA Kit (pS209) (ab277087)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 51 pg/ml
- Range: 234.38 pg/ml - 15000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human eIF4E ELISA Kit (pS209)
See all eIF4E kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.5% Inter-assay Sample n Mean SD CV% Extract 3 6.6% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
51 pg/ml -
Range
234.38 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 104 96% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human eIF4E ELISA kit (ab277087) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human eIF4E protein in human cell extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human eIF4E with 51 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
eIF-4E, Eukaryotic translation initiation factor 4E, and its complex components bind mRNA on its 7-methylguanosine-containing capped end and facilitate ribosomal interaction. This binding is the rate-limiting step to eukaryotic protein synthesis. Phosphorylation of serine-209 leads to a much tighter binding and increased protein synthesis. Upregulation of this protein has been shown to be associated with many forms of cancer. Antibodies for this kit were raised against non-modified full-length eIF-4E as well as a phosphorylated protein fragment encompassing the serine-209 site. This protein shares homology across Mammalia, and therefore may cross-react with many species. The protein standard in this product is recombinant human eIF-4E ligated with peptide containing phosphorylated Serine 209.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human eIF4E (pS209) Capture Antibody 1 x 600µl 10X Human eIF4E (pS209) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human eIF4E (pS209) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family. -
Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Alternative names
- AUTS19
- CBP
- eIF 4E
see all -
Database links
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human eIF4E (pS209) standard curve in 1X Cell Extraction Buffer PTR.The eIF4E (pS209) standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native eIF4E (pS209) in human HeLA cell extract based on a 20 µg/mL extract load.The concentrations of eIF4E (pS209) were measured in duplicate and interpolated from the eIF4E (pS209) standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean eIF4E (pS209) concentration was determined to be 9,702.98 pg/mL.
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Sandwich ELISA - Human eIF4E ELISA Kit (pS209)To learn more about the advantages of recombinant antibodies see here.
