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Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11935] to PNK/PNKP - C-terminal
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11935] - C-terminal
    See all PNK/PNKP primary antibodies

  • Description

    Rabbit monoclonal [EPR11935] to PNK/PNKP - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, PC-3, Jurkat and Human testis lysate. Human breast and pancreas tissue. HeLa cells.

  • General notes

     

     

     This product was previously labelled as PNK

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11935

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution.  Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

    Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.

  • Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
    Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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