Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR043F] to B7H4
Suitable for: ICC, IHC-P, IP, WB
Reacts with: Human

Product name

Anti-B7H4 antibody [BLR043F]
See all B7H4 primary antibodies
Description

Rabbit monoclonal [BLR043F] to B7H4
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human B7H4 (extracellular).
Database link: Q7Z7D3
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR043F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-B7H4 antibody [BLR043F] (ab243899)

B7H4 was immunoprecipitated from 1.0 mg OVCAR-4 whole cell lysate with ab243899 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243899 at 1/1000 dilution.
Lane 1: ab243899 IP in OVCAR-4 whole cell lysate.

Lane 2: Control IgG in OVCAR-4 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.
Western blot - Anti-B7H4 antibody [BLR043F] (ab243899)

Western blot analysis using ab243899 at 1/1000 dilution.
Lane 1: OVCAR-4 whole cell lysate (50 µg).
Lane 2: HepG2 whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HeLa whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
Lane 6: Jurkat whole cell lysate (50 µg).
Lane 7: MOLT-4 whole cell lysate (50 µg).
Lane 8: HH whole cell lysate (50 µg).
Lane 9: C5/MJ whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry - Anti-B7H4 antibody [BLR043F] (ab243899)

Formalin-fixed, paraffin-embedded NIH:OVCAR-3 (human ovary adenocarcinoma cell line) cells labeling B7H4 using ab243899 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-B7H4 antibody [BLR043F] (ab243899)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for B7H4 using ab243899 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-B7H4 antibody [BLR043F] (ab243899)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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