Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15771] to SPOP - C-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SPOP antibody [EPR15771] - C-terminal
    See all SPOP primary antibodies

  • Description

    Rabbit monoclonal [EPR15771] to SPOP - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, PC3, HCT116 and HepG2 whole cell lysate (ab7900).

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15771

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    All lanes : Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SPOP knockout HeLa cell lysate
    Lane 3 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab192233 observed at 49 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab192233 Anti-SPOP antibody [EPR15771] - C-terminal was shown to specifically react with SPOP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265634 (knockout cell lysate ab257702) was used. Wild-type and SPOP knockout samples were subjected to SDS-PAGE. ab192233 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    All lanes : Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa
    Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    Western blot - Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    Anti-SPOP antibody [EPR15771] - C-terminal (ab192233) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)
    Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-SPOP antibody [EPR15771] - C-terminal (ab192233)

© 2021 Astana Biomed Group LLP. All rights reserved.