Anti-GST3 / GST pi antibody (ab53943)
Key features and details
- Goat polyclonal to GST3 / GST pi
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GST3 / GST pi antibody
See all GST3 / GST pi primary antibodies -
Description
Goat polyclonal to GST3 / GST pi -
Host species
Goat -
Specificity
ab53943 is specific for GST pi. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Full length native protein (purified) GST3/ GST pi (Rat)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GST3 / GST pi antibody (ab53943)Amido Black stain and Western blot analysis of GST-π antibody.
Lane 1. Molecular weight marker.
Lane 2. Purified GST-π
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (ab53943)IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
