Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free
    See all Hemoglobin subunit beta/ba1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20614] to Hemoglobin subunit beta/ba1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

    Recombinant full length protein within Human Hemoglobin subunit beta/ba1 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P68871

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat heart lysate; Mouse heart and lung lysates.

  • General notes

    ab240390 is the carrier-free version of ab214049 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240390 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Hemoglobin subunit beta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20614

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)
    Immunoprecipitation - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)

    Hemoglobin subunit beta/ba1 was immunoprecipitated from 0.35 mg of TF-1 (human bone marrow erythroleukemia cell line) lysate with ab214049 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214049 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: TF-1 whole cell lysate 10 µg (Input). 

    Lane 2: ab214049 IP in TF-1 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214049 in TF-1 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214049).

  • Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)
    Western blot - Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)
    All lanes : Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] (ab214049) at 1/1000 dilution

    Lane 1 : Rat heart lysate at 20 µg
    Lane 2 : Mouse heart lysate at 20 µg
    Lane 3 : Mouse lung lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure times: Lane 1-2: 3 minutes; Lane 3: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214049).

  • Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)
    Anti-Hemoglobin subunit beta/ba1 antibody [EPR20614] - BSA and Azide free (ab240390)

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