Anti-hnRNP U/p120 antibody [3G6] (ab10297)
![Anti-hnRNP U/p120 antibody [3G6] (ab10297)](https://www.abcam.com/ps/products/10/ab10297/Images/ab10297-17887-anti-hnrnp-u-p120-antibody-3g6-western-blot.jpg "Anti-hnRNP U/p120 antibody [3G6] (ab10297)")
Key features and details
- Mouse monoclonal [3G6] to hnRNP U/p120
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-hnRNP U/p120 antibody [3G6]
See all hnRNP U/p120 primary antibodies -
Description
Mouse monoclonal [3G6] to hnRNP U/p120 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to hnRNP U/p120. RNPs eluted from an oligo (dt) cellulose column
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Positive control
- WB: HeLa cytoplasmic and nuclear fraction. HEK-293T whole cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue. Flow Cytometry: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3G6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-hnRNP U/p120 antibody [3G6] (ab10297)All lanes : Anti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/1000 dilution
Lane 1 : Cytoplasmic fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : Nuclear fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Predicted band size: 120 kDa
Observed band size: 120 kDa
This correlates with the information published in the reference Dreyfuss, G. et. al. -
![Western blot - Anti-hnRNP U/p120 antibody [3G6] (ab10297)](https://www.abcam.com/ps/products/10/ab10297/Images/ab10297-17885-anti-hnrnp-u-p120-antibody-3g6-western-blot.jpg)
Western blot - Anti-hnRNP U/p120 antibody [3G6] (ab10297) This image is courtesy of an Abreview submitted by Dr Stoil DmitrovAnti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/5000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 100 µg
Secondary
HRP conjugated sheep anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [3G6] (ab10297)](https://www.abcam.com/ps/products/10/ab10297/Images/hnRNP-U-Primary-antibodies-ab10297-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [3G6] (ab10297)ab10297 (4 µg/ml)) staining hnRNP U/p120 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of Purkinje cells neurons.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with a protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
![Flow Cytometry - Anti-hnRNP U/p120 antibody [3G6] (ab10297)](https://www.abcam.com/ps/products/10/ab10297/Images/hnRNP-U-Primary-antibodies-ab10297-3.jpg)
Flow Cytometry - Anti-hnRNP U/p120 antibody [3G6] (ab10297)Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab10297 (red line). The cells were fixed with 100% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10297, 1 µg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
