Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSG101 antibody [EPR7131(B)] (ab133586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7131(B)] to TSG101
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSG101 antibody [EPR7131(B)]
    See all TSG101 primary antibodies

  • Description

    Rabbit monoclonal [EPR7131(B)] to TSG101

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TSG101 (C terminal). The exact sequence is proprietary.
    Database link: Q99816

  • Positive control

    • WB: PC12, NIH 3T3, K562, 293T and Jurkat whole cell lysate (ab7899). IP: K562 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/2000 dilution (purified)

    Lane 1 : K562 whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : PC-12 whole cell lysate
    Lane 4 : NIH/3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    Blocking/Diluting buffer 5% NFDM/TBST
  • Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)

    ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).

    Lane 1 (input): K562 whole cell lysate 10μg

    Lane 2 (+): ab133586 + K562 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    All lanes : Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/1000 dilution (unpurified)

    Lane 1 : PC12 cell lysate
    Lane 2 : NIH 3T3 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 44 kDa



    Note: Dilute primary antibody in 1% BSA.
  • OI-RD Scanning - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    OI-RD Scanning - Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TSG101 antibody [EPR7131(B)] (ab133586)
    Anti-TSG101 antibody [EPR7131(B)] (ab133586)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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