Anti-TSG101 antibody (ab228013)
Key features and details
- Rabbit polyclonal to TSG101
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TSG101 antibody
See all TSG101 primary antibodies -
Description
Rabbit polyclonal to TSG101 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: HEK-293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. ICC/IF: A549 cells. IP: A549 whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TSG101 antibody (ab228013) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TSG101 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 44 kDa10% SDS-PAGE gel.
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All lanes : Anti-TSG101 antibody (ab228013) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 44 kDa10% SDS-PAGE gel.
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All lanes : Anti-TSG101 antibody (ab228013) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 44 kDa10% SDS-PAGE gel.
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All lanes : Anti-TSG101 antibody (ab228013) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbite IgG
Predicted band size: 44 kDa10% SDS-PAGE gel.
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TSG101 was immunoprecipitated from A549 (human lung carcinoma cell line from) whole cell extract with 5 µg of ab228013. Western blot was performed from the immunoprecipitate using ab228013. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instaed of ab228013 in A549 whole cell extract.
Lane 2: ab228013 IP in A549 whole cell extract.
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2% paraformaldehyde-fixed, 0.1% saponin/10% NGS/0.75% glycine-permeabilized A549 (human lung carcinoma cell line) cells stained for TSG101 (green) using ab228013 at 1/500 dilution in ICC/IF.
Nuclear counter stain: Hoechst 33342 (blue).