Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7130(B)] to TSG101
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TSG101 antibody [EPR7130(B)]
    See all TSG101 primary antibodies

  • Description

    Rabbit monoclonal [EPR7130(B)] to TSG101

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: K562, Jurkat, and SH-SY5Y cell lysates. Human brain lysate; mouse and rat heart tissue lysates. IHC-P: Human kidney tissue. ICC: HEK-293 cells. Flow Cyt: HeLa and K562 cells.

  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

    Lane 1 : Human brain lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Flow cytometric analysis of permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.

  • OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) (purified)

    All lanes : Mouse heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

    Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

  • Anti-TSG101 antibody [EPR7130(B)] (ab125011)
    Anti-TSG101 antibody [EPR7130(B)] (ab125011)

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