Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 147 417 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7130(B)] to TSG101
Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-TSG101 antibody [EPR7130(B)]
See all TSG101 primary antibodies
Description

Rabbit monoclonal [EPR7130(B)] to TSG101
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human TSG101 aa 1-100. The exact sequence is proprietary.
Positive control
- WB: K562, Jurkat, and SH-SY5Y cell lysates. Human brain lysate; mouse and rat heart tissue lysates. IHC-P: Human kidney tissue. ICC: HEK-293 cells. Flow Cyt: HeLa and K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 4.00 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7130(B)
Isotype

IgG
Research areas

Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Human brain lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TSG101 with unpurified ab125011 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometric analysis of permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling TSG101 (red) with unpurified ab125011 at 1/10. Green - Isotype control, rabbit monoclonal IgG.
OI-RD Scanning - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/10000 dilution (purified)

Lane 1 : Jurkat cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TSG101 with purified ab125011 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) at 1/500 dilution (unpurified)

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Immunocytochemsitry/Immunofluorescence analysis of HEK-293 (human epithelial cell line from embryonic kidney) cells labeling TSG101 (red) with purified ab125011 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with unpurified ab125011 at 1/3 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Western blot - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

All lanes : Anti-TSG101 antibody [EPR7130(B)] (ab125011) (purified)

All lanes : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Flow Cytometry - Anti-TSG101 antibody [EPR7130(B)] (ab125011)

Flow cytometry analysis of permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TSG101 with purified ab125011 at 1/40 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Anti-TSG101 antibody [EPR7130(B)] (ab125011)

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