Anti-Grp75/MOT antibody [N52A/42] (ab94668)
Key features and details
- Mouse monoclonal [N52A/42] to Grp75/MOT
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Grp75/MOT antibody [N52A/42]
See all Grp75/MOT primary antibodies -
Description
Mouse monoclonal [N52A/42] to Grp75/MOT -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Mouse Grp75/MOT aa 551-766.
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Positive control
- WB: HeLa cell lysate. ICC/IF Heat shocked HeLa cells.
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General notes
The clone number has been updated from S52A-42 to N52A/42, both clone numbers name the same antibody clone.
Previously labelled as Grp75.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N52A/42 -
Isotype
IgG1 -
Research areas
Images
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Anti-Grp75/MOT antibody [N52A/42] (ab94668) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP
Predicted band size: 74 kDaBlock: 1.5% BSA for 30 minutes at RT.
Incubated with primary antibody for 2 hours at RT.
Incubated with secondary antibody for 1 hour at RT.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Grp75/MOT (Green) using ab94668 at a 1/100 dilution in ICC/IF.
Heat shocked HeLa cells were fixed in 2% formaldehyde for 20 minutes at RT. Incubated with primary antibody for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT.