Anti-Grp75/MOT antibody (ab227215)
Key features and details
- Rabbit polyclonal to Grp75/MOT
- Suitable for: IP, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Grp75/MOT antibody
See all Grp75/MOT primary antibodies -
Description
Rabbit polyclonal to Grp75/MOT -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis
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Immunogen
Recombinant fragment within Human Grp75/MOT (internal sequence). The exact sequence is proprietary.
Database link: P38646 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2 and SCC-15 whole cell extract. IHC-P: Mouse duodenum tissue; rat duodenum and brain tissues. IP: HepG2 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Grp75/MOT antibody (ab227215)All lanes : Anti-Grp75/MOT antibody (ab227215) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab227215)Paraffin-embedded rat duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (ab227215)Immunocytochemistry/ Immunofluorescence of HeLa cells staining Grp75/MOT with ab227215 (1/500). Hoechst 33342 staining. (blue).
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Western blot - Anti-Grp75/MOT antibody (ab227215)Anti-Grp75/MOT antibody (ab227215) at 1/500 dilution + SCC-15 (Human squamous cell carcinoma, tongue) whole cell extract at 6 µg
Predicted band size: 74 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab227215)Paraffin-embedded mouse duodenum tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (ab227215)Paraffin-embedded rat brain tissue stained for Grp75/MOT using ab227215 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Grp75/MOT antibody (ab227215)Grp75/MOT was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab227215. Western blot was performed from the immunoprecipitate using ab227215. Anti-Rabbit IgG was used as secondary antibody.
Lane 1: Control IgG instead of ab227215 in HepG2 whole cell extract.
Lane 2: ab227215 IP in HepG2 whole cell extract.
