Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [236A/E7] to FOXP3 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FOXP3 antibody [236A/E7] - BSA and Azide free
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [236A/E7] to FOXP3 - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    The epitope recognized by FOXP3 antibody [236A/E7] (ab20034) is between amino acids 105-236. FOXP3 antibody [236A/E7] (ab20034) is expected to detect full length FOXP3 as well as both cleaved forms.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Fusion protein

  • Positive control

    • WB: Human mammary gland lysate, HEK-293 transfected with FOXP3 expression vector containing a GFP-Myc-tag, whole cell lysate. IHC-P: Human tonsil and thymus tissue.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 8th February 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048) at 1/1000 dilution + Human mammary gland lysate at 20 µg

    Predicted band size: 47 kDa



    This data was developed using ab20034, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)

    This data was developed using ab20034, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    All lanes : Anti-FOXP3 antibody [236A/E7] (ab20034) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty
    vector (vector control), containing a GFP-Myc-tag, whole cell lysate
    Lane 2 : HEK-293 transfected with FOXP3 (WT) expression vector containing a GFP-Myc-tag, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 47 kDa


    Exposure time: 1 second


    This data was developed using ab20034, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)

    This data was developed using ab20034, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling FOXP3 with ab23004 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat AntiRabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

  • Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    Western blot - Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)
    All lanes : Anti-FOXP3 antibody [236A/E7] (ab20034) at 5 µg/ml

    Lane 1 : HEK293T cell lysate
    Lane 2 : HEk293T cell lysate overexpressing Human FOXP3
    Lane 3 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 47 kDa



    This data was developed using ab20034, the same antibody clone in a different buffer formulation.

    ab20034 detects Human FOXP3 protein at ~50 kDa in HEK293T cells overexpressing the protein. It also detects FOXP3 in Human tonsil tissue lysate, however this band is significantly weaker in endogenous conditions. Upon higher exposure, weak bands can also be observed in HEK293T cell lysate.

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with Anti-FOXP3 antibody [236A/E7] (ab20034; 5 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-mouse (green; 1:10000) for 1 hour at room temperature before imaging.

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