Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15941] to Mint-1 - N-terminal
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Mint-1 antibody [EPR15941] - N-terminal
    See all Mint-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15941] to Mint-1 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, Human cerebellum, Human Transitional cell carcinoma of bladder.

  • General notes

     

     

    Previously labelled as APBA1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human transitional cell carcinoma of the bladder labeling Mint-1 with ab190359 at 1/50 dilution and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/50000 dilution + Human fetal brain at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 156 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/10000 dilution + Human cerebellum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa
    Observed band size: 156 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)

    Immunoprecipitation of Human fetal brain labeling Mint-1 with ab190359 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000

  • Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)
    Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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