Mouse Angiopoietin-like 4 ELISA Kit (ab210577)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 29 pg/ml
- Range: 117.2 pg/ml - 7500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Angiopoietin-like 4 ELISA Kit
See all Angiopoietin-like 4/ANGPTL4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 3.7% Inter-assay Sample n Mean SD CV% Supernatant 3 6.6% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
29 pg/ml -
Range
117.2 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 108 105% - 114% Cell culture media 103 98% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Angiopoietin-like 4 ELISA Kit (ab210577) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiopoietin-like 4 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Angiopoietin-like 4 with 29 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Angiopoietin-like 4 (Angiopoietin-related protein 4,ANGPTL4) is a member of the ANGPTL family and structurally related to angiopoietins. Angiopoietin-like 4 is secreted and may also associate with the extracellular matrix. It is associated with angiogenesis and metastasis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Angiopoietin-related protein 4 Capture Antibody 1 x 600µl 10X Mouse Angiopoietin-related protein 4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse Angiopoietin-related protein 4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
Tissue specificity
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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Alternative names
- Angiopoietin like 4
- Angiopoietin related protein 4
- Angiopoietin-like protein 4
see all -
Database links
- Entrez Gene: 57875 Mouse
- SwissProt: Q9Z1P8 Mouse
- Unigene: 196189 Mouse
Images
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Other - Mouse Angiopoietin-like 4 ELISA Kit (ab210577)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Mouse Angiopoietin-related protein 4 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of Mouse Angiopoietin-related protein 4 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Angiopoietin-related protein 4 in mouse cell and tissue culture supernatant samples.The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 20%, heart supernatant 50%, and NIH3T3 Supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 9,986 pg/mL in lung supernatant, 606 pg/mL in heart supernatant and 1,030 pg/mL in NIH3T3 cell culture supernatant.
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Interpolated concentrations of native Angiopoietin-related protein 4 in mouse liver extract sample based on a 2,000 µg/mL extract load.The concentrations of Angiopoietin-related protein 4 were measured in duplicate and interpolated from the Angiopoietin-related protein 4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 748 pg/mL in mouse liver extract.
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Interpolated concentrations of native Angiopoietin-related protein 4 in mouse tissue culture supernatant samples.The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 10,668 pg/mL in lung supernatant, 2,884pg/mL in liver supernatant, 2,703pg/mL in brain supernatant and 456 pg/mL in heart supernatant.
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Comparison of secreted Angiopoietin-related protein 4 in unstimulated and PDGF- stimulated NIH/3T3 supernatant.NIH/3T3 cells were grown in the absence or presence of Platelet Derived Growth Factor (PDGF) for 24 hours. The concentrations of Angiopoietin-related protein 4 were measured in duplicates, interpolated from the Angiopoietin-related protein 4 standard curves and corrected for sample dilution. Undiluted samples are 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiopoietin-related protein 4 concentration was determined to be 4,704 pg/mL in unstimulated, 20,700 pg/ml in PDGF stimulated and undetectable in media.
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Sandwich ELISA - Mouse Angiopoietin-like 4 ELISA Kit (ab210577)To learn more about the advantages of recombinant antibodies see here.
