Mouse Angiopoietin-like 4 Matched Antibody Pair Kit (ab216789)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
-
Product name
Mouse Angiopoietin-like 4 Matched Antibody Pair Kit
See all Angiopoietin-like 4/ANGPTL4 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
39.77 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Angiopoietin-like 4 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Angiopoietin-like 4.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210577. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Angiopoietin-like 4 Capture Antibody 2 x 50µg 1 x 50µg Mouse Angiopoietin-like 4 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Angiopoietin-like 4 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
Tissue specificity
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
-
Alternative names
- Angiopoietin like 4
- Angiopoietin related protein 4
- Angiopoietin-like protein 4
see all -
Database links
- Entrez Gene: 57875 Mouse
- SwissProt: Q9Z1P8 Mouse
- Unigene: 196189 Mouse
Images
-
Mouse Angiopoietin-like 4 standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Mouse Angiopoietin-like 4 Matched Antibody Pair Kit (ab216789)To learn more about the advantages of recombinant antibodies see here.
