Mouse Angiopoietin-like 4 Antibody Pair - BSA and Azide free (ab241769)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Angiopoietin-like 4 Antibody Pair - BSA and Azide free
See all Angiopoietin-like 4/ANGPTL4 kits -
Assay type
ELISA set -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse Angiopoietin-related protein 4. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab210577), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Angiopoietin-like 4 Capture Antibody (unconjugated) 1 x 100µg Mouse Angiopoietin-like 4 Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
Tissue specificity
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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Alternative names
- Angiopoietin like 4
- Angiopoietin related protein 4
- Angiopoietin-like protein 4
see all -
Database links
- Entrez Gene: 57875 Mouse
- SwissProt: Q9Z1P8 Mouse
- Unigene: 196189 Mouse
Images
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Sandwich ELISA - Mouse Angiopoietin-like 4 Antibody Pair - BSA and Azide free (ab241769)To learn more about the advantages of recombinant antibodies see here.
