Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Key features and details
- Rabbit polyclonal to TMEM49/VMP1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-TMEM49/VMP1 antibody - C-terminal
See all TMEM49/VMP1 primary antibodies -
Description
Rabbit polyclonal to TMEM49/VMP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human TMEM49/VMP1 aa 350 to the C-terminus (C terminal). NP_112200.2
Database link: Q96GC9 -
Positive control
- WB: Rat pancreas lysate. ICC/IF: RKO cells.
-
General notes
This product was previously labelled as TMEM49
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)
Immunocytochemistry/Immunofluorescence analysis of RKO (Human colon carcinoma cell line) cells labeling TMEM49/VMP1 using ab240887 at 1:100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain at 1:1000 dilution for 60 min at RT; DAPI (blue) nuclear stain at 1:5000 for 5 min at RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240887 (D) Composite.
-
Western blot - Anti-TMEM49/VMP1 antibody - C-terminal (ab240887)16 hours at 4°C at 1/1000 dilution + Rat pancreas cell lysate at 15 µg
Secondary
Goat-Anti-Rabbit IgG: HRP for 60 minutes at RT at 1/200 dilutionBlock: 5% skimmed milk in 1X TBST.
Color development: TMB.
