Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13304] to Carbonic Anhydrase 7/CA7
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304]
    See all Carbonic Anhydrase 7/CA7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13304] to Carbonic Anhydrase 7/CA7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and fetal kidney tissue lysates; 293 and Raw264.7 cell lysates; HeLa cells.

  • General notes

     

     

     This product was previously labelled as CA7

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13304

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    All lanes : Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/20000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution + Raw264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

    Immunofluorescence analysis of HeLa cells labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/50 dilution (4 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrol images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.

  • Immunoprecipitation - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Immunoprecipitation - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

    Immunoprecipitation analysis of Human fetal brain tissue lysate labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/20 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Human fetal brain tissue lysate.

  • Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)
    Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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