Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13304] to Carbonic Anhydrase 7/CA7
Suitable for: WB, ICC/IF, IP
Reacts with: Mouse, Human

Product name

Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304]
See all Carbonic Anhydrase 7/CA7 primary antibodies
Description

Rabbit monoclonal [EPR13304] to Carbonic Anhydrase 7/CA7
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain and fetal kidney tissue lysates; 293 and Raw264.7 cell lysates; HeLa cells.
General notes
This product was previously labelled as CA7

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13304
Isotype

IgG
Research areas

Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

All lanes : Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : 293 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa
Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/20000 dilution + Human fetal kidney lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa
Western blot - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408) at 1/5000 dilution + Raw264.7 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Immunofluorescence analysis of HeLa cells labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/50 dilution (4 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrol images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.
Immunoprecipitation - Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

Immunoprecipitation analysis of Human fetal brain tissue lysate labeling Carbonic Anhydrase 7/CA7 using ab191408 at 1/20 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Human fetal brain tissue lysate.
Anti-Carbonic Anhydrase 7/CA7 antibody [EPR13304] (ab191408)

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