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Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13753] to BRAT1 - C-terminal
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BRAT1 antibody [EPR13753] - C-terminal
    See all BRAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13753] to BRAT1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293 and jurkat cell lysate. 293 and HeLa cells.

  • General notes

     

     

     This product was previously labelled as BAAT1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    Western blot - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    All lanes : Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    Immunoprecipitation - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

    Western blot analysis of BRAT1 in immunoprecipitation pellets from Human Jurkat lysate. ab181855 used at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.

  • Flow Cytometry - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    Flow Cytometry - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

    Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling BRAT1 with ab181855 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control Rabbit IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    Immunocytochemistry/ Immunofluorescence - Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling BRAT1 with ab181855 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG(Alexa Fluor®488) at 1/200 dilution, Conterstained with Dapi blue.

  • Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)
    Anti-BRAT1 antibody [EPR13753] - C-terminal (ab181855)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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