PANC-1 nuclear extract lysate (ab14882)
Overview
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Product name
PANC-1 nuclear extract lysate -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
PANC-1 cells are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. The cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth-factor receptor 2 (HER2/neu) oncogene (which is present in 60-70% of human pancreatic carcinomas) but are estrogen receptor (ER) negative. Cells are also negative for MUC4 (Sialomucin complex SMC, an intramembrane ligand for ErbB2) and positive for Smad4 (a TGF beta signalling cascade protein inactivated in human gastrointestinal cancers).