TGF Beta FirePlex®-HT Panel (ab270739)
Overview
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Product name
TGF Beta FirePlex®-HT Panel -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Multiplex -
Species reactivity
Reacts with: Mouse, Rat, Human, Non human primates
Predicted to work with: Dog
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Product overview
TGF Beta FirePlex®-HT Panel (ab270739) uses FirePlex® particle technology to detect TGF-B1, TGF-B2, and TGF-B3 from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere.
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Notes
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
The following table provides the sensitivity, dynamic range, and CVs for TGF Beta FirePlex®-HT Panel 1 *.
Target Sensitivity/LOD
(pg/ml)
Dynamic Range
(pg/ml)
Inter-well CV
(Std curve)
TGF-B1 6.39 13.72 - 3,333 6.1% TGF-B2 48.63 123.46 - 30,000 5.0% TGF-B3 27.57 41.15 - 10,000 5.7% *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here. -
Platform
Multiplex
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 384 tests 384 Well Imaging Plate 1 unit 384 Well Imaging Plate Lid 1 unit 384 Well Imaging Plate Seal 2 units FirePlex-HT 1X PBS 1 x 8ml FirePlex-HT 5X Capture Particle Mix 1 x 700µl FirePlex-HT Protein Standard Mix HTTG 1 x 7 tests FirePlex-HT Reagent Diluent R2 1 x 8ml FirePlex-HT Sample Diluent S1 1 x 25ml FirePlex-HT - Imaging Dye Concentrate 1 x 875µl FirePlex-HT 35X Detector Antibody Mix 1 x 110µl -
Research areas
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Cellular localization
TGF beta 1: Secreted > extracellular space > extracellular matrix. TGF beta 2: Secreted. TGF beta 3: Secreted. -
Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 7042 Human
- Entrez Gene: 7043 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 21808 Mouse
- Entrez Gene: 21809 Mouse
- Entrez Gene: 25717 Rat
- Entrez Gene: 59086 Rat
see all
Images
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FirePlex-HT Assay Workflow
Assay Workflow
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FirePlex-HT Standard Curve PlotStandard Curve