Antibodies, Proteins, Kits and Reagents for Life Science

Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2697(2)] to AP2S1 - BSA and Azide free
Suitable for: IHC-P, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free
See all AP2S1 primary antibodies
Description

Rabbit monoclonal [EPR2697(2)] to AP2S1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast carcinoma, thyroid carcinoma and colon carcinoma tissues IP: Human fetal brain lysate
General notes
ab242393 is the carrier-free version of ab128950.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2697(2)
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

ab128950 (purified) at 1:60 dilution (2µg) immunoprecipitating AP2S1 in Human fetal brain lysate.
Lane 1 (input): Human fetal brain lysate 10µg
Lane 2 (+): ab128950 & Human fetal brain lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128950 in Human fetal brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128950).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded human colon carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128950).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-AP2S1 antibody [EPR2697(2)] - BSA and Azide free (ab242393)

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