Anti-gamma Adaptin antibody - N-terminal (ab205953)
Key features and details
- Rabbit polyclonal to gamma Adaptin - N-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-gamma Adaptin antibody - N-terminal
See all gamma Adaptin primary antibodies -
Description
Rabbit polyclonal to gamma Adaptin - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human gamma Adaptin aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O43747 -
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-gamma Adaptin antibody - N-terminal (ab205953)
Detection of gamma Adaptin in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205953 at 6 µg/mg lysate for IP and 0.4µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-gamma Adaptin antibody - N-terminal (ab205953)All lanes : Anti-gamma Adaptin antibody - N-terminal (ab205953) at 0.1 µg/ml
Lane 1 : TCMK1 whole cell lysate
Lane 2 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 seconds
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Western blot - Anti-gamma Adaptin antibody - N-terminal (ab205953)All lanes : Anti-gamma Adaptin antibody - N-terminal (ab205953) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 10 seconds
