Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2697(2)] to AP2S1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-AP2S1 antibody [EPR2697(2)]
    See all AP2S1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2697(2)] to AP2S1

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human AP2S1 aa 100-200. The exact sequence is proprietary.
    Database link: P53680

  • Positive control

    • WB: Human fetal brain, SH-SY5Y, HEK-293T, HeLa, Mouse brain, Rat brain lysates, Mouse kidney and Mouse brain lysates. IHC-P: Human breast carcinoma, thyroid carcinoma and colon carcinoma tissues IP: Human fetal brain lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2697(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    All lanes : Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution (Purified)

    Lane 1 : Human fetal brain lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Mouse kidney lysates
    Lane 6 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    14% gel is preferred to 4-20% gradient gel

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution (Purified) + Mouse kidney lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    All lanes : Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Immunoprecipitation - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    ab128950 (purified) at 1:60 dilution (2µg) immunoprecipitating AP2S1 in Human fetal brain lysate.
    Lane 1 (input): Human fetal brain lysate 10µg
    Lane 2 (+): ab128950 & Human fetal brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128950 in Human fetal brain lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Western blot - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    All lanes : Anti-AP2S1 antibody [EPR2697(2)] (ab128950) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : 293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    ab128950, at a 1/250 dilution, staining AP2S1 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling AP2S1 with purified ab128950 at 1:100 dilution (12.07 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-AP2S1 antibody [EPR2697(2)] (ab128950)
    Anti-AP2S1 antibody [EPR2697(2)] (ab128950)

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