Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22417] to PITX2/RGS - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free
    See all PITX2/RGS primary antibodies

  • Description

    Rabbit monoclonal [EPR22417] to PITX2/RGS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IHC-Fr or IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse and rat pituitary gland tissue.

  • General notes

    ab244343 is the carrier-free version of ab221142 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab244343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PITX2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

    Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

    Negative control.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control no staining on rat liver (PMID: 11157981) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

    Negative control.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control,no staining on mouse spleen (PMID: 11157981)  is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
    Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)

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