Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
![Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)](https://www.abcam.com/ps/products/244/ab244343/Images/ab244343-2-221142IHC2.jpg "Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22417] to PITX2/RGS - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free
See all PITX2/RGS primary antibodies -
Description
Rabbit monoclonal [EPR22417] to PITX2/RGS - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat pituitary gland tissue.
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General notes
ab244343 is the carrier-free version of ab221142 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab244343 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PITX2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22417 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)](https://www.abcam.com/ps/products/244/ab244343/Images/ab244343-1-221142IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)](https://www.abcam.com/ps/products/244/ab244343/Images/ab244343-4-221142IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)Negative control.
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control no staining on rat liver (PMID: 11157981) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)](https://www.abcam.com/ps/products/244/ab244343/Images/ab244343-3-221142IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)Negative control.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control,no staining on mouse spleen (PMID: 11157981) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221142).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)](https://www.abcam.com/ps/products/244/ab244343/Images/ab244343-5-benefits-of-recombinant-antibodies.png)
Anti-PITX2/RGS antibody [EPR22417] - BSA and Azide free (ab244343)
