Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23888-5] to BRD9 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free
    See all BRD9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23888-5] to BRD9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ChIP,Flow Cyt (Intra),ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, 293T, NIH/3T3, PC-12, K-562, HDLM-2, LNCaP, Jurkat, Neuro-2a and RAW 264.7, C6 lysates. IHC-P: Human testis, Mouse testis, Rat testis tissues.

  • General notes

    ab277488 is the carrier-free version of ab259839. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277488 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : HDLM-2 (human hodgkin lymphoma ) whole cell lysate
    Lane 3 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
    Lane 4 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 67 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259839, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates used in this blot have experienced freeze-thaw cycles.

     Exposure time: Lanes 1-2: 37 seconds;Lanes 3-4: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)

    This data was developed using ab259839, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lanes 1 & 5 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lanes 4 & 6 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 67 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab259839, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:32457312; 31015438 ).

    Lysates were made freshly and used in WB immediately to minimize protein degradation (lane1-4). Lanes 5-6 are the lysates from same cell lines but have experienced freeze-thaw cycles.

    Exposure time: Lanes 1-4: 37 seconds; Lanes 5-6: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)

    This data was developed using ab259839, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Western blot - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    All lanes : Anti-BRD9 antibody [EPR23888-5] (ab259839) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 2 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 3 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 67 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    This data was developed using ab259839, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates used in this blot have experienced freeze-thaw cycles.

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)

    This data was developed using ab259839, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling BRD9 with ab259839 at 1/500 dilution (0.908 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat testis. The section was incubated with ab259839 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)
    Anti-BRD9 antibody [EPR23888-5] - BSA and Azide free (ab277488)

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