Anti-acetyl Lysine antibody [RM101] (ab190479)
![Anti-acetyl Lysine antibody [RM101] (ab190479)](https://www.abcam.com/ps/products/190/ab190479/Images/ab190479-225785-anti-acetyl-lysine-antibody-rm101-immunoprecipitation.jpg "Anti-acetyl Lysine antibody [RM101] (ab190479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM101] to acetyl Lysine
- Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IF
- Reacts with: Species independent
Overview
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Product name
Anti-acetyl Lysine antibody [RM101]
See all acetyl Lysine primary antibodies -
Description
Rabbit monoclonal [RM101] to acetyl Lysine -
Host species
Rabbit -
Specificity
ab190479 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, or lysine with other modification. -
Tested applications
Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to acetyl Lysine conjugated to bovine serum albumin.
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Positive control
- A431 cells treated with Trichostatin A; HeLa whole cell lysate - Trichostatin A treated
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM101 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-acetyl Lysine antibody [RM101] (ab190479)
Lane 1: A431 whole cell lysate
Lane 2: A431 whole cell lysate (pretreated with Trichostatin A)
Lane 3: A431 whole cell lysate immunoprecipitated with Rabbit IgG
Lane 4: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with Rabbit IgG
Lane 5: A431 whole cell lysate immunoprecipitated with ab190479 at 1/500
Lane 6: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with ab190479 at 1/500Western blot performed using anti-PTEN mouse monoclonal antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)](https://www.abcam.com/ps/products/190/ab190479/Images/ab190479-273261-anti-acetyl-lysine-antibody-rm101-immunofluorescence.png)
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)Immunocytochemical staining of HeLa cells labelling Acetyl Lysine with ab190479 at 1:100. Actin filaments are labelled using fluorescein phalloidin (green), and nuclei are stained with DAPI (blue).
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![Western blot - Anti-acetyl Lysine antibody [RM101] (ab190479)](https://www.abcam.com/ps/products/190/ab190479/Images/ab190479-225784-anti-acetyl-lysine-antibody-rm101-western-blot.jpg)
Western blot - Anti-acetyl Lysine antibody [RM101] (ab190479)All lanes : Anti-acetyl Lysine antibody [RM101] (ab190479) at 1/2000 dilution
Lanes 1 & 3 & 5 : Lysate of nontreated HeLa cells
Lanes 2 & 4 & 6 : Lysate of HeLa cells treated with Trichostatin A
Developed using the ECL technique.Exposure time increased from blot on left (lanes 1, 2) to blot on right (lanes 5,6).
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![Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)](https://www.abcam.com/ps/products/190/ab190479/Images/ab190479-225783-anti-acetyl-lysine-antibody-rm101-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)Immunofluorescent analysis of A431cells nontreated (left) or treated with Trichostatin A (right), using ab190479 at 1/500 followed by a PE conjugated secondary antibody (red) and DAPI (blue).
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![Anti-acetyl Lysine antibody [RM101] (ab190479)](https://www.abcam.com/ps/products/190/ab190479/Images/ab190479-5-benefits-of-recombinant-antibodies.png)
Anti-acetyl Lysine antibody [RM101] (ab190479)
