Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-acetyl Lysine antibody [RM101] (ab190479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM101] to acetyl Lysine
  • Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IF
  • Reacts with: Species independent

Overview

  • Product name

    Anti-acetyl Lysine antibody [RM101]
    See all acetyl Lysine primary antibodies

  • Description

    Rabbit monoclonal [RM101] to acetyl Lysine

  • Host species

    Rabbit

  • Specificity

    ab190479 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, or lysine with other modification.

  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide corresponding to acetyl Lysine conjugated to bovine serum albumin.

  • Positive control

    • A431 cells treated with Trichostatin A; HeLa whole cell lysate - Trichostatin A treated

Properties

Images

  • Immunoprecipitation - Anti-acetyl Lysine antibody [RM101] (ab190479)
    Immunoprecipitation - Anti-acetyl Lysine antibody [RM101] (ab190479)

    Lane 1: A431 whole cell lysate

    Lane 2: A431 whole cell lysate (pretreated with Trichostatin A)

    Lane 3: A431 whole cell lysate immunoprecipitated with Rabbit IgG

    Lane 4: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with Rabbit IgG

    Lane 5: A431 whole cell lysate immunoprecipitated with ab190479 at 1/500
    Lane 6: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with ab190479 at 1/500

     

    Western blot performed using anti-PTEN mouse monoclonal antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)
    Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)

    Immunocytochemical staining of HeLa cells labelling Acetyl Lysine with ab190479 at 1:100. Actin filaments are labelled using fluorescein phalloidin (green), and nuclei are stained with DAPI (blue).

  • Western blot - Anti-acetyl Lysine antibody [RM101] (ab190479)
    Western blot - Anti-acetyl Lysine antibody [RM101] (ab190479)
    All lanes : Anti-acetyl Lysine antibody [RM101] (ab190479) at 1/2000 dilution

    Lanes 1 & 3 & 5 : Lysate of nontreated HeLa cells
    Lanes 2 & 4 & 6 : Lysate of HeLa cells treated with Trichostatin A

    Developed using the ECL technique.


    Exposure time increased from blot on left (lanes 1, 2) to blot on right (lanes 5,6).

  • Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)
    Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (ab190479)

    Immunofluorescent analysis of A431cells nontreated (left) or treated with Trichostatin A (right), using ab190479 at 1/500 followed by a PE conjugated secondary antibody (red) and DAPI (blue).

  • Anti-acetyl Lysine antibody [RM101] (ab190479)
    Anti-acetyl Lysine antibody [RM101] (ab190479)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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