Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free
Suitable for: WB, IP, IHC-P, ChIP-sequencing, ICC/IF
Reacts with: Human

Product name

Anti-Brd4 antibody [BL-149-2H5] - BSA free
See all Brd4 primary antibodies
Description

Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ChIP-seq	Human
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Brd4 aa 1312-1362. The exact sequence is proprietary. NP_490597.1 and Gene ID 23476.
Database link: O60885
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272042 is the carrier-free version of ab243862. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-149-2H5
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).
Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for brd4 using ab243862 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).
Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 µL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

Lane 1: ab243862 IP in HeLa cell lysate.

Lane 2: Contol IgG in HeLa cell lysate.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).
Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

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