Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free
  • Suitable for: WB, IP, IHC-P, ChIP-sequencing, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Brd4 antibody [BL-149-2H5] - BSA free
    See all Brd4 primary antibodies

  • Description

    Rabbit monoclonal [BL-149-2H5] to Brd4 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP-seq
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Brd4 aa 1312-1362. The exact sequence is proprietary. NP_490597.1 and Gene ID 23476.
    Database link: O60885

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272042 is the carrier-free version of ab243862. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

Properties

Images

  • ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    ChIP-sequencing - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq using ab243862.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

  • Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Immunocytochemistry/ Immunofluorescence - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling brd4 using ab243862 at 1/250 dilution in ICC/IF analysis. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for brd4 using ab243862 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

  • Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Immunoprecipitation - Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

    brd4 was immunoprecipitated from 1 mg HeLa whole cell lysate with ab243862 at 20 µL/mg lysate. Western blot was performed on the immunoprecipitate using ab243862 at 1/1000 dilution.

    Lane 1: ab243862 IP in HeLa cell lysate.

    Lane 2: Contol IgG in HeLa cell lysate.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243862).

  • Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)
    Anti-Brd4 antibody [BL-149-2H5] - BSA free (ab272042)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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